Equipment

 

The facility currently operates:

MoFlo Astrios EQ

MoFlo Astrios EQ for high-speed cell sorting, equipped with 6 lasers (405, 488, 532, 561, 592, and 640nm) and 28 detectors is housed in a biosafety cabinet and is capable of 6-way sorting.

MoFlo Astrios (6 Laser/ 28 detector) configuration and commonly used fluorophores :

astriosgraph

LSR-II Analyser

4-laser (405, 488, 561 and 640 nm) 12-detector with a high throughput sampler for multiparameter flow analysis.

LSR-II (4 Laser/13 color) configuration and commonly used fluorophores :

lsriigraph

Aria-561

Aria-561 for high-speed cell sorting, equipped with 5 lasers (355 (UV), 407, 488, 561 and 633 nm) and 16 detectors for up to 15-color discrimination, and 5-way sorting.

Aria -561 (5 Laser/15 color) configuration and commonly used fluorophores :

ariagraph

FACSymphony

The BD FACSymphony is a 5 laser, 28 color analyzer with high throughput sampler for very high parameter flow analysis

FACSymphony (5 Laser/28 color) configuration and commonly used fluorophores :

symphony

Aria-594 - Researcher Assisted

For high speed cell sorting.
Staff will always be present to help with sorting, but researchers may need to help monitor/change tubes if all staff members are busy with the other sorters.

Researcher Assisted Aria-564 (4 Laser/ 14 detector) Configuration and commonly used fluorophores :

symphony

FACS-Canto

2-laser (488 and 633 nm) 8-detector with 40 tube automated carousel loader.

FACSCanto (2 Laser/6 color) configuration and commonly used fluorophores :