MBIB

Flow Cytometry Core Facility

The Division of Immunology’s Flow Cytometry Facility provides flow cytometric analysis and cell sorting services to Division and Harvard Stem Cell Institute investigators. It also welcomes users from Harvard Medical School and affiliated institutions. The Facility is operationally connected to the Flow Cytometry Core at the Joslin Diabetes Center.

The facility currently operates:
(1) LSR-II analyzer (3-laser, 12-detector) with a high throughput sampler for multiparameter flow analysis;
(2) FACS-Calibur for routine 4-color analysis;
(3) FACSAria for high-speed cell sorting, equipped with 4 lasers (including UV) and 16 detectors for up to 14-color discrimination, sorting in tubes or plates, and 4-way sorting, under sterile conditions.
(4) MoFlo Astrios EQ for high-speed cell sorting, equipped with 6 lasers (405, 488, 532, 561, 592, and 640nm) and 28 detectors is housed in a biosafety cabinet and is capable of 6-way sorting.
(5) FACSCanto (2-laser, 10-detector) with a 40 tube carousel loader for multiparameter flow analysis.

Facility staff  advise and train investigators in the applications of flow cytometry and use of the instruments, and help with study design and choice of fluorochrome combinations. Sorting on the Aria is primarily performed by Chad Araneo with the possibility of “self-operation” for highly trained and experienced users). The analyzers are operated directly by the investigators, after training and certification by facility staff.