Flow Cytometry Core Facility

The Division of Immunology’s Flow Cytometry Facility provides flow cytometric analysis and cell sorting services to Division and Harvard Stem Cell Institute investigators. It also welcomes users from Harvard Medical School and affiliated institutions. The Facility is operationally connected to the Flow Cytometry Core at the Joslin Diabetes Center.

We are pleased to announce that we have expanded our analysis capabilities at the Immunology Division flow core with the addition of a BD FACSymphony A5 28 color analyzer.
The Symphony is the newest generation BD instrument on the market offering many exciting possibilities. 5 lasers and 30 detectors means more information can be collected per cell. Maximizing data collected on rare samples and allowing for deep phenotyping. All new low-noise electronics results in higher resolution for dim populations compared to LSR or Fortessa. Highly expandable and customizable in the future with room for 10 lasers and 52 detectors.

The facility also currently operates:
(1) LSR-II analyzer (4-laser, 12-detector) with a high throughput sampler for multiparameter flow analysis;
(2) Aria-561 for high-speed cell sorting, equipped with 5 lasers (including UV) and 17 detectors for up to 15-color discrimination, sorting in tubes or plates, and 4-way sorting, under sterile conditions.
(3) Aria-594 for high-speed cell sorting, equipped with 4 lasers and 14 detectors for up to 12-color discrimination, sorting in tubes or plates, and 4-way sorting, under sterile conditions.
(4) MoFlo Astrios EQ for high-speed cell sorting, equipped with 6 lasers (405, 488, 532, 561, 592, and 640nm) and 28 detectors is housed in a biosafety cabinet and is capable of 6-way sorting.
(5) FACSCanto (2-laser, 10-detector) with a 40 tube carousel loader for multiparameter flow analysis.

Facility staff  advise and train investigators in the applications of flow cytometry and use of the instruments, and help with study design and choice of fluorochrome combinations. Sorting on the Aria is primarily performed by Chad Araneo with the possibility of “self-operation” for highly trained and experienced users). The analyzers are operated directly by the investigators, after training and certification by facility staff.

 

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