MBIB

Operations

 

Location

Room 938D
New Research Building
77, Avenue Louis Pasteur, Boston, MA 02115

Booking

Hours of Operation :
Monday, Wednesday, Thursday, Friday:  9:00am-5:00pm
Tuesday:   12:00pm-8:00pm

Please login here to book time on the equipment, which is open 2 weeks in advance.

Please note that cancellations must be at least 48 hours in advance to avoid charges.

Training

Please e-mail Chad ( Chad_Araneo@hms.harvard.edu) to arrange training prior to booking all equipment with the exception of FACSAria and MoFlo Atrios EQ.

Sort Sample Requirements

- Cell concentration not to exceed 107 cells/mL
- Cells are to be filtered through 45 uM mesh prior to sorting (after final wash or staining step)
- Cells can be brought in 12x75 mm round bottom tubes or 15 mL conical Tubes
- Phenol red is not recommended in cell suspension media.
- An unstained control and a single positive control for each fluorophore used is required.

Collection Tube Requirements

Tube Sorting : All collection tubes should be filled approximately ¼  with desired buffer (cell growth media, PBS, lysis buffer etc). Eppendorf tubes or 12x75 mm round bottom tubes can be used for  1, 2, 3 or 4 way sorting, 15 mL conical tubes can be used for 1 or 2 way sorting.
Plate Sorting : Cells can be sorted directly into 6, 12, 24, 48, or 96 well plates. Wells should contain appropriate  buffer.
Please indicate plate type on the calendar at the time of sign up.

Pricing

Rates for use of Cytometry (per hour) :

 

ARIA-561

LSR-II

ASTRIOS

ARIA-594

FACSymphony

Canto

Harvard faculty

$75

$45

$75

$75

$45

$36

All External Academic Users
(Including Affiliate Institutions)

$83

$53

$83

$83

$53

$44

Commercial Users

$153

$123

$153

$153

$123

$114